FreezeFrame 4 - Actimetrics
FreezeFrame 4 - Actimetrics
FreezeFrame 4: Video System for Fear Conditioning – Tail Suspension – Porsolt Swim Test
FreezeFrame, the first video-based system for fear conditioning, has become the standard in its field. It combines the sensitivity of the human observer with the objectivity and high throughput of automation. While infrared beams can detect gross movements of an animal from one part of the cage to another, FreezeFrame can detect the minute movements of grooming, sniffing, turning and rearing. And FreezeFrame monitors the animal at up to 15 times per second, not once every 5 seconds, for far more objective and reproducible results. FreezeFrame can also collect and process data for learned helplessness experiments, including Tail Suspension and the Porsolt Forced Swim Test.
- FreezeFrame now supports fear conditioning in optogenetic experiments
- FreezeFrame now ships together with LimeLight at no extra cost. LimeLight is our general-purpose tracking program for Open Field, Plus Maze, Radial Arm Maze and Novel Object Recognition and WaterMaze. Together FreezeFrame and LightLight provide automated video tracking for a complete range of rodent behavioral experiments.
- Complete systems available, including software, cameras, control interface, cages, isolation cubicles, shockers, audio amplifiers and cue and house lights.
Not a tracking system, but a proprietary motion detection algorithm filters out shadows, light flicker and camera noise, and detects movements as small as 1 mm.
Takashi Kitamura, Sachie K. Ogawa, Dheeraj S. Roy, Teruhiro Okuyama, Mark D. Morrissey, Lillian M. Smith, Roger L. Redondo, Susumu Tonegawa (2017) Engrams and circuits crucial for systems consolidation of a memory. Science 6333:73-78.
Dheeraj S. Roy, Autumn Arons, Teryn I. Mitchell, Michele Pignatelli, Tom s J. Ryan, Susumu Tonegawa (2016) Memory retrieval by activating engram cells in mouse models of early Alzheimer s disease. Nature 531,508 512.
Tom s J. Ryan, Dheeraj S. Roy, Michele Pignatelli, Autumn Arons, Susumu Tonegawa (2015) Engram cells retain memory under retrograde amnesia. Science 6238:1007-1013
Ainsley, J.A., Drane, L., Jacobs, J., Kittelberger, K.A., and Reijmers, L.G. (2014). Functionally diverse dendritic mRNAs rapidly associate with ribosomes following a novel experience. Nature communications 5, 4510.
Liu, C.C., Tsai, C.W., Deak, F., Rogers, J., Penuliar, M., Sung, Y.M., Maher, J.N., Fu, Y., Li, X., Xu, H., et al. (2014). Deficiency in LRP6-mediated Wnt signaling contributes to synaptic abnormalities and amyloid pathology in Alzheimer’s disease. Neuron 84, 63-77.
Jahrling, J.B., Hernandez, C.M., Denner, L., and Dineley, K.T. (2014). PPARgamma recruitment to active ERK during memory consolidation is required for Alzheimer’s disease-related cognitive enhancement. Journal of neuroscience 34, 4054-4063.
Heckman, L.D., Chahrour, M.H., and Zoghbi, H.Y. (2014). Rett-causing mutations reveal two domains critical for MeCP2 function and for toxicity in MECP2 duplication syndrome mice. eLife 3:e02676
Chen, T., Lu, J.S., Song, Q., Liu, M.G., Koga, K., Descalzi, G., Li, Y.Q., and Zhuo, M. (2014). Pharmacological rescue of cortical synaptic and network potentiation in a mouse model for fragile X syndrome. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 39, 1955-1967.
Gomez, A.M., Froemke, R.C., and Burden, S.J. (2014). Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4. eLife 3:e04287.
Ramirez S, Liu X, Lin PA, Suh J, Pignatelli M, Redondo RL, Ryan TJ, Tonegawa S. (2013). Creating a false memory in the hippocampus. Science 341:387-91.
Trouche S1, Sasaki JM, Tu T, Reijmers LG. (2013) Fear extinction causes target-specific remodeling of perisomatic inhibitory synapses. Neuron. 80:1054-65.
Parkhurst CN, Yang G, Ninan I, Savas JN, Yates JR, Lafaille JJ, Hempstead BL, Littman DR, Gan WB. (2013) Microglia promote learning-dependent synapse formation through brain-derived neurotrophic factor. Cell. 155:1596-1609.
A. S. Sengar, J. Ellegood, A. P. Yiu, H. Wang, W. Wang, S. C. Juneja, J. P. Lerch, S. A. Josselyn, R. M. Henkelman, M. W. Salter, and S. E. Egan (2103) Vertebrate Intersectin1 Is Repurposed to Facilitate Cortical Midline Connectivity and Higher Order Cognition The Journal of Neuroscience 33: 4055-4065.
Qi Shi, Marguerite Prior, Xiangdong Zhou, Xiaoying Tang, Wanxia He, Xiangyou Hu, and Riqiang Yan (2013) Preventing Formation of Reticulon 3 Immunoreactive Dystrophic Neurites Improves Cognitive Function in Mice. The Journal of Neuroscience 33: 3059-3066.
Carmelina Gemma, Matthew Fister, Charles Hudson, Paula C. Bickford (2013) Improvement of memory for context by inhibition of caspase-1 in aged rats. European Journal of Neuroscience22:1751 1756.
Miho Terunuma, Raquel Revilla-Sanchez, Isabel M. Quadros, Qiudong Deng, Tarek Z. Deeb, Michael Lumb, Piotr Sicinski, Philip G. Haydon, Menelas N. Pangalos, and Stephen J. Moss (2013) Postsynaptic GABAB Receptor Activity Regulates Excitatory Neuronal Architecture and Spatial Memory The Journal of Neuroscience 34: 804-816.
Liu, X., Ramirez, S., Pang, P.T., Puryear, C.B., Govindarajan, A., Deisseroth, K., and Tonegawa, S. (2012). Optogenetic stimulation of a hippocampal engram activates fear memory recall.Nature 484, 381-385.
Lugo, J.N., Brewster, A.L., Spencer, C.M., and Anderson, A.E. (2012). Kv4.2 knockout mice have hippocampal-dependent learning and memory deficits. Learning and Memory 19:182-189.
Song, C., Detert, J.A., Sehgal, M., and Moyer, J.R., Jr. (2012). Trace fear conditioning enhances synaptic and intrinsic plasticity in rat hippocampus. Journal of Neurophysiology 107:3397-3408.
Akers, K.G., Arruda-Carvalho, M., Josselyn, S.A., and Frankland, P.W. (2012). Ontogeny of contextual fear memory formation, specificity, and persistence in mice.Learning and Memory19, 598-604.
Kheirbek, M.A., Tannenholz, L., and Hen, R. (2012). NR2B-dependent plasticity of adult-born granule cells is necessary for context discrimination. Journal of Neuroscience 32:8696-8702.
Tanokashira, D., Morita, T., Hayashi, K., Mayanagi, T., Fukumoto, K., Kubota, Y., Yamashita, T., and Sobue, K. (2012). Glucocorticoid suppresses dendritic spine development mediated by down-regulation of caldesmon expression. J Neurosci 32, 14583-14591.
Kleschevnikov, A.M., Belichenko, P.V., Faizi, M., Jacobs, L.F., Htun, K., Shamloo, M., and Mobley, W.C. (2012). Deficits in cognition and synaptic plasticity in a mouse model of Down syndrome ameliorated by GABAB receptor antagonists. J Neurosci 32, 9217-9227.
Elder, G.A., Dorr, N.P., De Gasperi, R., Gama Sosa, M.A., Shaughness, M.C., Maudlin-Jeronimo, E., Hall, A.A., McCarron, R.M., and Ahlers, S.T. (2012). Blast exposure induces post-traumatic stress disorder-related traits in a rat model of mild traumatic brain injury. Journal of Neurotrauma 29, 2564-2575.
Lai, C.S., Franke, T.F., and Gan, W.B. (2012). Opposite effects of fear conditioning and extinction on dendritic spine remodelling. Nature 483, 87-91.
Parker, C.C., Sokoloff, G., Cheng, R., and Palmer, A.A. (2012). Genome-wide association for fear conditioning in an advanced intercross mouse line. Behavior Genetics 42, 437-448.
Sahay, A., Scobie, K.N., Hill, A.S., O’Carroll, C.M., Kheirbek, M.A., Burghardt, N.S., Fenton, A.A., Dranovsky, A., and Hen, R. (2011). Increasing adult hippocampal neurogenesis is sufficient to improve pattern separation. Nature 472, 466-470.
John J. Perkowski and Geoffrey G. Murphy (2011) Deletion of the Mouse Homolog of KCNAB2, a Gene Linked to Monosomy 1p36, Results in Associative Memory Impairments and Amygdala Hyperexcitability. Journal of Neuroscience 31:46-54.
Luke W Ayers, Galen Missig, Jay Schulkin and Jeffrey B Rosen (2011) Oxytocin Reduces Background Anxiety in a Fear-Potentiated Startle Paradigm: Peripheral vs Central AdministrationNeuropsychopharmacology (epub).
Kimberly N. Scobie, Benjamin J. Hall, Scott A. Wilke, Kristen C. Klemenhagen, Yoshiaki Fujii-Kuriyama, Anirvan Ghosh, Ren Hen and Amar Sahay (2011) Kr ppel-Like Factor 9 Is Necessary for Late-Phase Neuronal Maturation in the Developing Dentate Gyrus and during Adult Hippocampal Neurogenesis. Journal of Neuroscience 29:9875-9887.
Caterina M. Hernandez, Rakez Kayed, Hui Zheng, J. David Sweatt, and Kelly T. Dineley (2010) Loss of 7 Nicotinic Receptors Enhances -Amyloid Oligomer Accumulation, Exacerbating Early-Stage Cognitive Decline and Septohippocampal Pathology in a Mouse Model of Alzheimer’s Disease. Journal of Neuroscience 30:2442 2453.
Laleh Sinai, Steven Duffy and John C. Roder (2010) Src inhibition reduces NR2B surface expression and synaptic plasticity in the amygdala. Learning and Memory 17:364-371.
Yair Shemesh, Ada Eban-Rothschild, Mira Cohen, and Guy Bloch (2010) Molecular Dynamics and Social Regulation of Context-Dependent Plasticity in the Circadian Clockwork of the Honey Bee. Journal of Neuroscience 30:12517-12525.
Yu Ohmura, Takeshi Izumi, Taku Yamaguchi, Iku Tsutsui-Kimura, Takayuki Yoshida and Mitsuhiro Yoshioka (2010) The Serotonergic Projection from the Median Raphe Nucleus to the Ventral Hippocampus is Involved in the Retrieval of Fear Memory Through the Corticotropin-Releasing Factor Type 2 Receptor. Neuropsychopharmacology 35:1271 1278.
Ji Y Lee, Patricio T Huerta, Jie Zhang, Czeslawa Kowal, Eva Bertini, Bruce T Volpe & Betty Diamond (2009) Neurotoxic autoantibodies mediate congenital cortical impairment of offspring in maternal lupus. Nature Medicine 15:91-96.
Viviane Labrie, Steven Duffy, Wei Wang, Steven W. Barger, Glen B. Baker and John C. Roder (2009) Genetic inactivation of D-amino acid oxidase enhances extinction and reversal learning in mice. Learning and Memory 16:28-37.
Wataru Ito, Bing-Xing Pan, Chao Yang, Siddarth Thakur and Alexei Morozov (2009) Enhanced generalization of auditory conditioned fear in juvenile mice. Learning and Memory 16:187-192.
Bridget M. McKay, Elizabeth A. Matthews, Fernando A. Oliveira, and John F. Disterhoft (2009) Intrinsic Neuronal Excitability Is Reversibly Altered by a Single Experience in Fear Conditioning. Journal of Neurophysiology 102:2763-2770.
J-.H. Han, S. A. Kushner, A. P. Yiu, H-.L. Hsiang, T. Buch, A. Waisman, B. Bontempi, R.L. Neve, P.W. Frankland, and S. A. Josselyn (2009) Selective Erasure of a Fear Memory. Science 13: 323:1492-1496.
Daniela D. Pollak,Francisco J. Monje,Lee Zuckerman, Christine A. Denny, Michael R. Drew and Eric R. Kandel (2008) An Animal Model of a Behavioral Intervention for Depression. Neuron60:149-161.
Brandon C. McKinney, Wilson Sze, Jessica A. White, and Geoffrey G. Murphy (2008) L-type voltage-gated calcium channels in conditioned fear: A genetic and pharmacological analysis.Learning and Memory 15: 326-334
Ji Y Lee, Patricio T Huerta, Jie Zhang, Czeslawa Kowal, Eva Bertini, Bruce T Volpe and Betty Diamond (2008) Neurotoxic autoantibodies mediate congenital cortical impairment of offspring in maternal lupus. Nature Medicine 15:91 – 96.
Robert Waltereit, S nke Mannhardt, Sabine Nescholta, Christiane Maser-Gluth, and Dusan Bartsch (2008) Selective and protracted effect of nifedipine on fear memory extinction correlates with induced stress response. Learning and Memory 15:348-356.
Bing-Xing Pan, Francois Vautier, Wataru Ito, Vadim Y. Bolshakov, and Alexei Morozov (2008) Enhanced Cortico-Amygdala Efficacy and Suppressed Fear in Absence of Rap1. Journal of Neuroscience 28:2089-2098.
Albert Y. Hung, Kensuke Futai, Carlo Sala, Juli G. Valtschanoff, Jubin Ryu, Mollie A. Woodworth, Fleur L. Kidd, Clifford C. Sung, Tsuyoshi Miyakawa, Mark F. Bear, Richard J. Weinberg, and Morgan Sheng (2008) Smaller Dendritic Spines, Weaker Synaptic Transmission, but Enhanced Spatial Learning in Mice Lacking Shank1. Journal of Neuroscience28:1697 1708.
Naoki Matsuo, Leon Reijmers, Mark Mayford (2008) Spine-Type Specific Recruitment of Newly Synthesized AMPA Receptors with Learning. Science 319: 1104-1107.
Leon G. Reijmers, Brian L. Perkins, Naoki Matsuo, Mark Mayford (2007) Localization of a Stable Neural Correlate of Associative Memory Science 317:1230-1233.
T.J. Kotti, D.M.O. Ramirez, B.E. Pfeiffer, K.M. Huber and D.W. Russell (2006) Brain cholesterol turnover required for geranylgeraniol production and learning in mice. PNAS 103:3869-3847.
M.D. Saxe, F. Battaglia, J.-W. Wang, G. Malleret, D.J. David, J.E. Monckton, A.D.R. Garcia, M.V. Sofroniew, E.R. Kandel, Luca Santarelli, R. Hen and M.R. Drew (2006) Ablation of hippocampal neurogenesis impairs contextual fear conditioning and synaptic plasticity in the dentate gyrus. PNAS 103:17501-17506.
S. Shalin, C. Hernandez, M. Dougherty, D. Morrison, J. Sweatt (2006) Kinase Suppressor of Ras1 Compartmentalizes Hippocampal Signal Transduction and Subserves Synaptic Plasticity and Memory Formation. Neuron, 50:765-779.
J.M. Long, P. LaPorte, S. Merscher, B. Funke, B. Saint-Jore, A. Puech, R. Kucherlapati, B.E. Morrow, A.I. Skoultchi and A. Wynshaw-Boris (2006) Behavior of mice with mutations in the conserved region deleted in velocardiofacial/DiGeorge syndrome. Neurogenetics, 7:247-257.
B.C. McKinney and G.G. Murphy (2006) The L-Type voltage-gated calcium channel Cav1.3 mediates consolidation, but not extinction, of contextually conditioned fear in mice. Learning and Memory, 13:584-589.
C.A. Saura, G. Chen, S. Malkani, S.-Y. Choi, R.H. Takahashi, D. Zhang, G.K. Gouras, A. Kirkwood, R.G.M. Morris and J. Shen (2006) Conditional Inactivation of Presenilin 1 Prevents Amyloid Accumulation and Temporarily Rescues Contextual and Spatial Working Memory Impairments in Amyloid Precursor Protein Transgenic Mice. Journal of Neuroscience, 25:6755 6764.
L.G. Reijmers, J.K. Coats, M.T. Pletcher, T. Wiltshire, L.M. Tarantino and M. Mayford (2006) A mutant mouse with a highly specific contextual fear-conditioning deficit found in an N-ethyl-N-nitrosourea (ENU) mutagenesis screen. Learning and Memory, 13:143-149
Gleb P. Shumyatsky, Ga l Malleret, Ryong-Moon Shin, Shuichi Takizawa, Keith Tully, Evgeny Tsvetkov, Stanislav S. Zakharenko, Jamie Joseph, Svetlana Vronskaya, DeQi Yin, Ulrich K. Schubart, Eric R. Kandel, and Vadim Y. Bolshakov, (2005) stathmin, A gene enriched in the amygdala, controls both learned and innate fear Cell, 123:697-709.
Leon G. Reijmers, Jennifer K. Coats, Mathew T. Pletcher, Tim Wiltshire, Lisa M. Tarantino and Mark Mayford (2006) A mutant mouse with a highly specific contextual fear-conditioning deficit found in an N-ethyl-N-nitrosourea (ENU) mutagenesis screen. Learning and Memory 13:143-149.
Saura CA, Choi SY, Beglopoulos V, Malkani S, Zhang D, Shankaranarayana Rao BS, Chattarji S, Kelleher RJ 3rd, Kandel ER, Duff K, Kirkwood A, Shen J. (2005) Loss of presenilin function causes impairments of memory and synaptic plasticity followed by age-dependent neurodegeneration.Neuron 42:23-36.
Michelle L. Gottsch, Hongkui Zeng, John G. Hohmann, David Weinshenker, Donald K Clifton, and Robert A. Steiner (2005) Phenotypic Analysis of Mice Deficient in the Type 2 Galanin Receptor (GALR2). Molecular and Cellular Biology 25:4804-4811
Carlos A. Saura, Guiquan Chen, Seema Malkani, Se-Young Choi, Reisuke H. Takahashi, Dawei Zhang, Gunnar K. Gouras, Alfredo Kirkwood, Richard G. M. Morris and Jie Shen (2005) Conditional Inactivation of Presenilin 1 Prevents Amyloid Accumulation and Temporarily Rescues Contextual and Spatial Working Memory Impairments in Amyloid Precursor Protein Transgenic Mice Journal of Neuroscience 25:6755-6764
X Zhou, J M Long, M A Geyer, E Masliah, J R Kelsoe, A Wynshaw-Boris, K R Chien (2005) Reduced expression of the Sp4 gene in mice causes deficits in sensorimotor gating and memory associated with hippocampal vacuolization. Molecular Psychiatry 10:393-406.
MG Zhao, H Toyoda, SW Ko, HK Ding, LJ Wu, M Zhuo (2005) Deficits in Trace Fear Memory and Long-Term Potentiation in a Mouse Model for Fragile. Journal of Neuroscience 25: 7385-7392.
Kiyohiko Angata, Jeffrey M. Long, Olena Bukalo, Wenjau Lee, Alexander Dityatev, Anthony Wynshaw-Boris, Melitta Schachner, Minoru Fukuda|, and Jamey D. Marth (2004) Sialyltransferase ST8Sia-II Assembles a Subset of Polysialic Acid That Directs Hippocampal Axonal Targeting and Promotes Fear Behavior. J. Biol. Chem., 279:32603-32613.
Mesches, M.H., Gemma, C., Veng, L.M., Allgeier, C., Young, D.A., Browning, M.D. and Bickford, P.C. (2004) Sulindac improves memory and increases NMDA receptor subunits in aged Fischer 344 rats. Neurobiology of Aging 25:315-324.
Korzus, E., Rosenfeld, M.G. and Mayford, M (2004) CBP Histone Acetyltransferase Activity Is a Critical Component of Memory Consolidation. Neuron 42:961-972.
Mark D. Lindner, Donald B. Hodges, Jr., John B. Hogan, Anitra F. Orie, Jason A. Corsa, Donna M. Barten, Craig Polson, Barbara J. Robertson, Valerie L. Guss, Kevin W. Gillman, John E. Starrett, Jr., and Valentin K. Gribkoff (2003) An Assessment of the Effects of Serotonin 6 (5-HT6) Receptor Antagonists in Rodent Models of Learning. Journal of Pharmacology And Experimental Therapeutics 307:682-691.
Miller S, Yasuda M, Coats JK, Jones Y, Martone ME, Mayford M. (2002) Disruption of dendritic translation of CaMKIIalpha impairs stabilization of synaptic plasticity and memory consolidation.Neuron 36:507-19.